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celltrace violet (ctv) cell proliferation dye  (Thermo Fisher)


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    Thermo Fisher celltrace violet (ctv) cell proliferation dye
    Celltrace Violet (Ctv) Cell Proliferation Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+violet+(ctv)+cell+proliferation+dye/celltrace+violet/pm39053944-58-10-16
    Average 90 stars, based on 1 article reviews
    celltrace violet (ctv) cell proliferation dye - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Enhanced IL-15-mediated NK cell activation and proliferation by an ADAM17 function-blocking antibody involves CD16A, CD137, and accessory cells.
    Article Snippet: PBMCs or enriched NK cells, either unlabeled or labeled with CellTrace Violet (CTV) Cell Proliferation Dye (ThermoFisher Scientific) as per the manufacturer’s instructions, were cultured for up to 7 days at 37°C in 5% CO2 in RPMI 1640 media (Gibco) supplemented with 10% Heat Inactivated FBS (Gibco) and 1x Anti- Anti (Gibco), in the presence or absence of rhIL- 15 or rhIL- 2 (R&D Systems, Minneapolis, Minnesota, USA) and the indicated antibodies.

    Article Title: Prostaglandin E₂ impacts multiple stages of the natural killer cell antitumor immune response.
    Article Snippet: To assess degranulation, target cells were labeled with CellTrace Violet (CTV) cell proliferation dye (Invitrogen) and cultured with NK cells at a 1:1 ratio for 4 h at 37°C in complete RPMI-1640 medium containing GolgiStop and GolgiPlug (1/1000; BD Biosciences) and PE-Cy5 anti-CD107a mAb (H4A3; BD Biosciences) or isotype-matched control (IgG1; MOPC-21; BD Biosciences).

    Article Title: Highly potent quinoxalinediones inhibit α-hemolysin and ameliorate Staphylococcus aureus lung infections.
    Article Snippet: Sorted naı̈ve CD4+ T cells were stained with the proliferation dye CellTrace Violet (CTV, Thermo Fisher Scientific) before cultivation.

    Article Title: Enhanced IL-15-mediated NK cell activation and proliferation by an ADAM17 function-blocking antibody involves CD16A, CD137, and accessory cells
    Article Snippet: PBMCs or enriched NK cells, either unlabeled or labeled with CellTrace Violet (CTV) Cell Proliferation Dye (ThermoFisher Scientific) as per the manufacturer’s instructions, were cultured for up to 7 days at 37°C in 5% CO 2 in RPMI 1640 media (Gibco) supplemented with 10% Heat Inactivated FBS (Gibco) and 1x Anti-Anti (Gibco), in the presence or absence of rhIL-15 or rhIL-2 (R&D Systems, Minneapolis, Minnesota, USA) and the indicated antibodies.

    Article Title: Particle elasticity influences polymeric artificial antigen presenting cell effectiveness in vivo via CD8+ T cell activation, macrophage uptake, and the protein corona
    Article Snippet: Adoptive cell therapy (ACT) is an immunotherapy strategy for cancer that has seen widespread clinical success.. During ACT, patient-derived lymphocytes are stimulated with the antigen of interest ex vivo, proliferated, then returned to the patient to initiate an antigen-specific antitumor response.. While effective, this process is resource-intensive and logistically impossible for many patients.

    Labeling:

    Article Title: Depletion of alloreactive B cells by drug-resistant chimeric alloantigen receptor T cells to prevent transplant rejection.
    Article Snippet: Crossmatch scores were determined by using values 1 (%10%), 2 (10%–20%), 3 (20%–40%), 6 (40%–80%), and 8 (80%–100%) for increasing frequencies of dead cells according to standard protocols of the National Institutes of Health (USA).8 Reporter assay to determine CORA receptor signaling CORA receptors were transduced into a previously described Jurkatbased reporter cell line9 by addition of lentiviral particles in a multiplicities of infections (MOIs) of 1 and 5 mg/mL Polybrene Infection/ Transfection Reagent (Merck, Darmstadt, Germany) using spinoculation. .. After 48 h, transduced reporter cells were co-cultured with the indicated target cells previously labeled with CellTrace violet proliferation dye (CTV; Thermo Fisher Scientific) for 24 h in an effector to target (E:T) ratio of 1:1. ..



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    celltrace violet cell proliferation dye ctv  (Thermo Fisher)
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    (A – D) BM cells collected from tumor-free C57Bl/6J mice were cultured in vitro in the presence of granulocyte-macrophage colony stimulating factor GM-CSF (20 ng/mL) and E0771 conditioned media (CM) (50% V/V) for 4 days in the presence of Veh or PEP to generate MDSCs. (A) Experimental outline and gating scheme. (B) Representative flow plot of CD11b + Gr-1 + cells in Veh- or PEP-treated BM cultures. (C) Frequencies and (D) absolute cell numbers of CD11b + Gr-1 + cells (n = 4 mice). Shown are Means ± SEM. Unpaired t-test (*P < 0.05, ***P < 0.005). (E) Ccr2 gene expression in M-MDSC isolated from the spleens (sM-MDSC) of E0771 tumor-bearing mice and treated ex vivo with PEP (20 nM) or Veh for 72h as analyzed by qRT-PCR (n = 4 mice). Shown are Means ± SEM. Unpaired t-test (****P < 0.001). (F–I) Splenic CD11b + Gr-1 + MDSCs (sMDSCs) were isolated from E0771 tumor-bearing mice and co-cultured with <t>CellTrace</t> Violet (CTV)-labeled CD8 + T cells isolated from the spleens of tumor-free mice at a 1:2 T cell/MDSC ratio. sMDSCs were pre-treated with PEP (20 nM) or Veh for 3h prior to the addition of T cells and Dynabeads (anti-CD28/CD3). MDSC suppressive properties were assessed by CD8 + T cell activation (IFNγ expression) and <t>proliferation</t> (CTV dilution). (F) Representative contour plots and (G) IFNγ expression in CD8 + T cells (n = 3 mice). One-way ANOVA with multiple comparisons with Tukey correction (***P < 0.0001). (H–I) sPMN-MDSCs and sM-MDSCs were isolated from the spleens of tumor-bearing mice as previously described. Percent of CD8 + T cell proliferation after coculture with Veh- or PEP (20 nM)-pre-treated (H) sPMN-MDSCs and (I) sM-MDSCs at the indicated T cell:MDSC ratios (n = 3 mice). Shown are Means ± SEM. (**P < 0.01, ***P < 0.005).
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    (A – D) BM cells collected from tumor-free C57Bl/6J mice were cultured in vitro in the presence of granulocyte-macrophage colony stimulating factor GM-CSF (20 ng/mL) and E0771 conditioned media (CM) (50% V/V) for 4 days in the presence of Veh or PEP to generate MDSCs. (A) Experimental outline and gating scheme. (B) Representative flow plot of CD11b + Gr-1 + cells in Veh- or PEP-treated BM cultures. (C) Frequencies and (D) absolute cell numbers of CD11b + Gr-1 + cells (n = 4 mice). Shown are Means ± SEM. Unpaired t-test (*P < 0.05, ***P < 0.005). (E) Ccr2 gene expression in M-MDSC isolated from the spleens (sM-MDSC) of E0771 tumor-bearing mice and treated ex vivo with PEP (20 nM) or Veh for 72h as analyzed by qRT-PCR (n = 4 mice). Shown are Means ± SEM. Unpaired t-test (****P < 0.001). (F–I) Splenic CD11b + Gr-1 + MDSCs (sMDSCs) were isolated from E0771 tumor-bearing mice and co-cultured with <t>CellTrace</t> Violet (CTV)-labeled CD8 + T cells isolated from the spleens of tumor-free mice at a 1:2 T cell/MDSC ratio. sMDSCs were pre-treated with PEP (20 nM) or Veh for 3h prior to the addition of T cells and Dynabeads (anti-CD28/CD3). MDSC suppressive properties were assessed by CD8 + T cell activation (IFNγ expression) and <t>proliferation</t> (CTV dilution). (F) Representative contour plots and (G) IFNγ expression in CD8 + T cells (n = 3 mice). One-way ANOVA with multiple comparisons with Tukey correction (***P < 0.0001). (H–I) sPMN-MDSCs and sM-MDSCs were isolated from the spleens of tumor-bearing mice as previously described. Percent of CD8 + T cell proliferation after coculture with Veh- or PEP (20 nM)-pre-treated (H) sPMN-MDSCs and (I) sM-MDSCs at the indicated T cell:MDSC ratios (n = 3 mice). Shown are Means ± SEM. (**P < 0.01, ***P < 0.005).
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    (A – D) BM cells collected from tumor-free C57Bl/6J mice were cultured in vitro in the presence of granulocyte-macrophage colony stimulating factor GM-CSF (20 ng/mL) and E0771 conditioned media (CM) (50% V/V) for 4 days in the presence of Veh or PEP to generate MDSCs. (A) Experimental outline and gating scheme. (B) Representative flow plot of CD11b + Gr-1 + cells in Veh- or PEP-treated BM cultures. (C) Frequencies and (D) absolute cell numbers of CD11b + Gr-1 + cells (n = 4 mice). Shown are Means ± SEM. Unpaired t-test (*P < 0.05, ***P < 0.005). (E) Ccr2 gene expression in M-MDSC isolated from the spleens (sM-MDSC) of E0771 tumor-bearing mice and treated ex vivo with PEP (20 nM) or Veh for 72h as analyzed by qRT-PCR (n = 4 mice). Shown are Means ± SEM. Unpaired t-test (****P < 0.001). (F–I) Splenic CD11b + Gr-1 + MDSCs (sMDSCs) were isolated from E0771 tumor-bearing mice and co-cultured with CellTrace Violet (CTV)-labeled CD8 + T cells isolated from the spleens of tumor-free mice at a 1:2 T cell/MDSC ratio. sMDSCs were pre-treated with PEP (20 nM) or Veh for 3h prior to the addition of T cells and Dynabeads (anti-CD28/CD3). MDSC suppressive properties were assessed by CD8 + T cell activation (IFNγ expression) and proliferation (CTV dilution). (F) Representative contour plots and (G) IFNγ expression in CD8 + T cells (n = 3 mice). One-way ANOVA with multiple comparisons with Tukey correction (***P < 0.0001). (H–I) sPMN-MDSCs and sM-MDSCs were isolated from the spleens of tumor-bearing mice as previously described. Percent of CD8 + T cell proliferation after coculture with Veh- or PEP (20 nM)-pre-treated (H) sPMN-MDSCs and (I) sM-MDSCs at the indicated T cell:MDSC ratios (n = 3 mice). Shown are Means ± SEM. (**P < 0.01, ***P < 0.005).

    Journal: Cancer letters

    Article Title: PKC agonism restricts innate immune suppression, promotes antigen cross-presentation and synergizes with agonistic CD40 antibody therapy to activate CD8 + T cells in breast cancer

    doi: 10.1016/j.canlet.2022.01.017

    Figure Lengend Snippet: (A – D) BM cells collected from tumor-free C57Bl/6J mice were cultured in vitro in the presence of granulocyte-macrophage colony stimulating factor GM-CSF (20 ng/mL) and E0771 conditioned media (CM) (50% V/V) for 4 days in the presence of Veh or PEP to generate MDSCs. (A) Experimental outline and gating scheme. (B) Representative flow plot of CD11b + Gr-1 + cells in Veh- or PEP-treated BM cultures. (C) Frequencies and (D) absolute cell numbers of CD11b + Gr-1 + cells (n = 4 mice). Shown are Means ± SEM. Unpaired t-test (*P < 0.05, ***P < 0.005). (E) Ccr2 gene expression in M-MDSC isolated from the spleens (sM-MDSC) of E0771 tumor-bearing mice and treated ex vivo with PEP (20 nM) or Veh for 72h as analyzed by qRT-PCR (n = 4 mice). Shown are Means ± SEM. Unpaired t-test (****P < 0.001). (F–I) Splenic CD11b + Gr-1 + MDSCs (sMDSCs) were isolated from E0771 tumor-bearing mice and co-cultured with CellTrace Violet (CTV)-labeled CD8 + T cells isolated from the spleens of tumor-free mice at a 1:2 T cell/MDSC ratio. sMDSCs were pre-treated with PEP (20 nM) or Veh for 3h prior to the addition of T cells and Dynabeads (anti-CD28/CD3). MDSC suppressive properties were assessed by CD8 + T cell activation (IFNγ expression) and proliferation (CTV dilution). (F) Representative contour plots and (G) IFNγ expression in CD8 + T cells (n = 3 mice). One-way ANOVA with multiple comparisons with Tukey correction (***P < 0.0001). (H–I) sPMN-MDSCs and sM-MDSCs were isolated from the spleens of tumor-bearing mice as previously described. Percent of CD8 + T cell proliferation after coculture with Veh- or PEP (20 nM)-pre-treated (H) sPMN-MDSCs and (I) sM-MDSCs at the indicated T cell:MDSC ratios (n = 3 mice). Shown are Means ± SEM. (**P < 0.01, ***P < 0.005).

    Article Snippet: Isolated CD8 + T cells were labeled with CellTrace Violet Cell Proliferation Dye (CTV, Thermo Fisher) and were added to MDSCs (5×10 4 CD8 + T cells/well).

    Techniques: Cell Culture, In Vitro, Gene Expression, Isolation, Ex Vivo, Quantitative RT-PCR, Labeling, Activation Assay, Expressing